Device

Part:BBa_K1092003:Design

Designed by:   Group: iGEM13_Hong_Kong_CUHK   (2013-09-15)


T7-RBS-Dioxygenase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 176
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We codon optimized catechol 1,2-dioxygenase (EC 1.13.11.1) gene from Pseudomonas putida KT 2400.

Source

Gene synthesis

References

[1] Cao, B., Geng, A. and Lou, K., 2008. Induction of ortho- and meta-cleavage pathways in Pseudomonas in biodegradation of high benzoate concentration: MS identification of catabolic enzymes. Applied Microbiology and Biotechnology, 81(1), pp. 99-107.

[2] Kim, Y.H., Cho, K., Yun, S., Kim, J.Y., Kwon, K., Yoo, J.S. and Kim, S.I., 2006. Analysis of aromatic catabolic pathways in Pseudomonas putida KT 2440 using a combined proteomic approach: 2-DE/MS and cleavable isotope-coded affinity tag analysis. Proteomics, 6(4), pp. 1301-1318.

[3] Hadibarata, T. and Kristanti, R.A., 2012. Identification of metabolites from benzo[a]pyrene oxidation by ligninolytic enzymes of Polyporus sp. S133. Journal of environmental management, 111(0), pp. 115-119.

[4] Naiem H., Nadaf, Jai S. Ghosh., 2011. Purification and characterizationof catechol 1, 2-dioxygenase from Rhodococcus sp. NCIM 2891. Research Journal of Environmental and Earth Sciences, 3(5),pp. 608-613.

[5] Rosenberg D. 1998. Methods for analyzing trend data. In: Analytic Methods in Maternal and Child Health. Editors: Handler, A, Rosenberg, D, Monahan, C, Kennelly, J. Washington DC: Maternal and Child Health Bureau, HRSA, DHHS, pp. 191–231.